Journal: The Journal of Experimental Medicine

Article Title: The Runx1 Transcription Factor Inhibits the Differentiation of Naive CD4 + T Cells into the Th2 Lineage by Repressing GATA3 Expression

doi: 10.1084/jem.20021200

Figure Lengend Snippet: Effect of cotransduction of GATA3 and Runx1 on Th2 cell differentiation. (A) Naive CD4 + T cells were isolated from the spleens of wild-type and GATA3 -transgenic mice, TCR-stimulated, infected by retroviruses carrying pMX or pMX-Runx1, and cultured in the presence of IL-2. The GFP + population was sorted and reactivated via TCR. The cytokines secreted into the culture supernatant were measured by ELISA. The values obtained for the pMX-Runx1–infected cells are presented as percent inhibition of secretion, taking the values observed for the pMX-infected cells to be 100%. (B) Naive CD4 + T cells were isolated from the spleens of IL-4R (−/−) mice, TCR-stimulated, coinfected by pMX-GFP and pMX-rat CD2 retroviruses as indicated, and cultured in the presence of IL-2. The rat CD2 + population was sorted, TCR-stimulated, and processed for flow cytometrical analysis of intracellular IL-4. Data are representative of two independent experiments.

Article Snippet: Anti-rat CD2 PE (LFA-2) was purchased from Cedarlane.

Techniques: Cell Differentiation, Isolation, Transgenic Assay, Infection, Cell Culture, Enzyme-linked Immunosorbent Assay, Inhibition

Journal:

Article Title: Effects of cyclosporin A and a rapamycin derivative (SAR943) on chronic allergic inflammation in sensitized rats

doi: 10.1046/j.1365-2567.2003.01672.x

Figure Lengend Snippet: Effects of cyclosporin A (CsA) and SAR943 on CD2+ (a), CD4+ (b) and CD8+ (c) T-cell recruitment to the airways following repeated challenge with allergen. There was a significant increase in the number of (a) CD2+ (**P < 0·01) T cells and (b) CD4+ (*P < 0·05) T cells around the airways following repeated allergen exposure of rats compared with repeated saline exposure. Neither CsA nor SAR943 attenuated the recruitment of CD2+ T cells to the airways as compared with the vehicle-treated group (a). SAR943, but not CsA, attenuated the recruitment of CD4+ T cells to the airways, as compared with the vehicle-treated group (#P < 0·05) (b). Neither CsA nor SAR943 altered CD8+ T-cell recruitment compared with vehicle-treated rats. Data are expressed as mean values ± standard error of the mean (SEM).

Article Snippet: For staining CD2 + , CD4 + or CD8 + T lymphocytes in tissue sections, the sections were incubated with mouse anti-rat CD2, CD4 or CD8 mAb (pan T-cell markers; PharMingen, Cambridge Bioscience, Cambridge, UK).

Techniques: